Adaptive Immunity

Genetic Engineering

Gene Tech Packet

Lesson

Topic

Statement(s) and Objective(s)

Activities

1	Techniques	3.5.U4: Genetic modification is carried out by gene transfer between species. Outline how the universality of the genetic code allows for gene transfer between species. 3.5.A2: Gene transfer in bacteria using plasmids makes use of restriction endonucleases and DNA ligases. Describe a technique for genetic modification including plasmids, restriction enzymes, reverse transcriptase and ligase. Outline why plasmids with genes coding for antibiotic resistance are chosen as vectors in gene transfer between species.	Introduction to genetic engineering Plasmid engineering handwritten notes Plasmid engineering dance Recombinant DNA POGIL Genetic engineering with plasmid models Example 1 Example 2 Example 3 Modeling plasmid engineering (key) pGLO transformation student intro Intro slides Hypothesis statements pGLO plasmid Plasmid map Plasmid sequence Bio Rad manual Teacher prep summary Student manual Procedure slides Quick guide example results data analysis Protein purification extension Protein purification manual SitC Lighting Life
2	Examples	2.7.A2: Production of human insulin in bacteria as an example of the universality of the genetic code allowing gene transfer between species. Outline the source and use of pharmaceutical insulin prior to the use of gene transfer technology. Outline the benefits of using gene transfer technology in the production of pharmaceutical insulin.	Application of gene transfer tech notes How did they make insulin from recombinant DNA? Biotech Newspapers Assignment directions Scoring checklist doc Peer scoring form Example A&B: living factories of the future A&B: Rice genetically engineered to resist heat waves can also produce up to 20% more grain Scitable: recombinant DNA tech Scitable: Engineering biofuel Scitable: transgenic animals in ag Scitable: transgenic crops Scitable: many examples of GMO
3	Assessing risks	3.5.NOS: Assessing risks associated with scientific research- scientists attempt to assess the risks associated with genetically modified crops or livestock. State two ways in which the risk of scientific research can be assessed. 3.5.A3: Assessment of potential risks and benefits associated with genetic modification of crops. Outline potential environmental, health and agricultural benefits and risks associated with genetic modification of crops. Assess the risks and benefits of an example of a genetically modified crop (i.e. golden rice). 3.5.S3: Analysis of data on risks to monarch butterflies of Bt crops. Outline the formation and use of Bt crops in agriculture. Assess the impact of Bt corn on monarch butterflies.	GMO pros and cons (sorted) Bioethics Seminar directions / teacher guide GATTACA movie questions Analyzing Risks to Monarchs slides Scitable: use and impact of bt maize A&B: Too much of a good thing podcast A&B: Migrating monarchs podcast A&B: GMO… OMG? Podcast (listen 17:50-24:40) UK government urged to consider changing law to allow gene editing of embryos \| Genetics \| The Guardian Course Source Published lesson Presentation slides Pre-reading rubric Does it pose a threat questions pt 1 Does it pose a threat questions pt 2 Summarize main claims task Summary of papers
4	CRISPR-Cas 9 Resources:		National Geographic CRISPR TED talk CRISPR SitC: TEDed: CRISPR in the Classroom RadioLab CRISPR-Cas9
5	Wrap Up and Review		Final knowledge audit Quizizz review Review slides